Transposon Induced Nitrogenase in Rhizobium japonicum Infecting Vigna radiata
نویسندگان
چکیده
منابع مشابه
Transposon Tn5-induced mutagenesis of Rhizobium japonicum yielding a wide variety of mutants.
When the "suicide" vector pSUP1011, which carries transposon Tn5 (Kmr), was introduced into Rhizobium japonicum USDA 110, kanamycin-resistant (Kmr) colonies were detected at a frequency (4.2 X 10-6) ca. 30 times greater than the spontaneous kanamycin resistance frequency (1.4 X 10-7). Ten thousand Kmr mutants were isolated and tested for nutritional auxotrophy. Auxotrophs were detected at a fre...
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Rhizobium strains nodulating green gram [Vigna radiata (L.) Wilczek] were found to produce bacteriocin on modified Bergersen's medium and inhibited the growth of homologous Rhizobium strains. Four bacteriocin producing and four bacteriocin non-producing strains were compared for their effect on nodulation, in planta nitrogenase activity and plant dry weight of green gram. The bacteriocin produc...
متن کاملMung Bean (Vigna radiata) Seedlings'
Two forms of Fd-NADP+ oxidoreductase (FNR) isoproteins have been purified and characterized from the first foliage leaves of 5d-old mung bean (Vigna radiata). They could be separated by either Mono Q HR 515 or ferredoxin (Fd)-Sepharose 48 affinity columns. Based on immunoblot analysis and N-terminal amino acid sequences, one form resembles the FNR purified from photosynthetic tissues of higher ...
متن کاملExtracellular polysaccharide composition, ex planta nitrogenase activity, and DNA homology in Rhizobium japonicum.
The composition of the major acidic extracellular polysaccharide (EPS) of 25 strains of Rhizobium japonicum was determined. Eight strains synthesized an acidic EPS containing rhamnose and 4-O- methylglucuronic acid and were closely related according to DNA homology. These same strains also expressed high levels of ex planta nitrogenase activity. Sixteen strains produced an acidic EPS containing...
متن کاملL-Arabinose metabolism in Rhizobium japonicum.
l-Arabinose was metabolized through an oxidative pathway by extracts of a strain of Rhizobium japonicum. The findings showed that l-arabinose is converted into 2-keto-3-deoxy-l-arabonate, which is cleaved into glycoaldehyde and pyruvate.
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ژورنال
عنوان ژورنال: Journal of Biotechnology & Biomaterials
سال: 2017
ISSN: 2155-952X
DOI: 10.4172/2155-952x.1000252